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Writer panagene     (Date : 2008-04-27 22:19:14)
Subject Which PNA probes form triple helices?
Content

PNA probes normally form double helices, unless the PNA probe contains a long pyrimidine stretch. For PNA probes that are homopyrimidine (5 or more pyrimidines in length) or contain 7 or more pyrimidine nucleobases in a row hybridization may produce a triple helix. It is also important to note that PNA probes can hybridize in both the parallel and anti-parallel orientations, unlike DNA oligomers that will only hybridize in the anti-parallel orientation.


 

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