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J. Clin. Microbiol. doi:10.1128/JCM.01398-08
Copyright (c) 2009, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.
A Novel PNA based Array for Detecting and Genotyping Human Papillomaviruses
Jae-jin Choi, Chunhee Kim, and Heekyung Park*
PANAGENE Inc., Daejeon 305-510, Korea
* To whom correspondence should be addressed. Email: hkpark@panagene.com
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Abstract
We describe a novel array for accurate and reliable genotyping of HPV using Peptide Nucleic Acid (PNA) probes. In order to exploit the superior hybridization properties of PNA with target HPV DNAs, we developed a novel PNA array (PANArrayTM HPV). PANArrayTM HPV enables detection and genotyping of HPVs using 32 type-specific PNA capture probes for medically important HPVs. All tested HPV types showed a highly unique hybridization pattern with type-specific PNA probes. PNA array results showed stable specificities and sensitivities up to 13-months storage at room temperature. And, we demonstrated the superior specificity, sensitivity and stability of PNA arrays for HPV genotyping. We compared the genotyping results of the PNA array to sequencing with MY09/11 PCR products derived from 72 clinical samples. The results showed an excellent agreement between the PNA array and sequencing, except for samples reflecting multiple infections. The results from PNA array were compared with those of type-specific PCR when discrepant results occurred owing to multiple infections. The results for PNA array matched those of type-specific PCR in all cases. Newly developed PNA arrays show excellent specificity, sensitivity and long shelf-life. Our results suggest that the PNA array represents a reliable alternative to conventional DNA array for HPV genotyping as well as for diagnostics.
http://jcm.asm.org/cgi/content/abstract/JCM.01398-08v1
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