Nucleic Acids Symp Ser (Oxf). 2009;(53):175-6.

Handy and prompt DNA separation using PNA with internal disulfide bond.

Aiba Y, Komiyama M.
Research Center for Advanced Science and Technology, The University of Tokyo, Tokyo 153-8904, Japan.

PNA (peptide nucleic acid) is a DNA analogue which has a peptide backbone. PNA is very useful as a recognition probe because of its immensely high affinity to DNA. In this study, we used PNA as a DNA separation tool. Furthermore, we introduced a cleavable disulfide bond into main chain of PNA in order to facilitate the removal of PNA from PNA/DNA duplex. This disulfide bond was readily cleaved by various reducing agents and then the resultant two short PNA fragments could not form stable PNA/DNA duplex anymore. Accordingly, a desired DNA fragment was picked up from the DNA mixtures and readily recovered by the reductive treatment.

PMID: 19749317 [PubMed - in process]

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