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BRAF mutation is found in several cancers including thyroid cancer, malignant melanoma, ovarian cancer, colorectal cancer. In case of thyroid cancer, there are over 40 different types of BRAF mutation. Of these mutations, the V600E mutation on exon 15, i.e. change of nucleotide from T to A in 1799th base that results in change from valine to glutamate in amino acids, is most important with over 90% of frequency. Especially, BRAF V600E mutation is detected with ca. 45% of frequency in papillary thyroid carcinoma and is considered a prognostic maker for thyroid cancer.
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As this product is Medical Device, please read 'caution' and 'instruction' before use.
Deliberation number : 2013-I10-11-2364 |
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Product name
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Cat. No.
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Size
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PNAClamp⑩ BRAF Mutation Detection Kit
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PNAC-2001 (5 mutations)
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50 tests
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· PNAC-2001 (5 mutations)
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No.
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Reagent
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Exon
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Amino Acid Change
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Nucleotide Change
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Cosmic No.
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1
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BRAF PNA mix #2
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15
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p.V600E
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c.1799T>A
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476
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p.V600D
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c.1799_1800 TG>AT
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477
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p.V600K
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c.1798_1799 GT>AA
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473
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p.V600R
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c.1798_1799 GT>AG
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474
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p.V600A
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c.1799T>C
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18443
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| Cosmic No. is taken from the Catalogue of Somatic Mutations in Cancer. (http://www.sanger.ac.uk/genetics/CGP/cosmic) |
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 Ready-to-use kit with result available within 3hours.
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Compatible Real Time PCR
- ABI 7500, ABI 7900, ABI Step one plus, Bio-Rad CFX96, Light Cycler480 (Roche), Rotor-Gene Q.
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Compatible Sample
- Fresh tissue, FFPE tissue.
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Test Several Biomarkers in One Plate with same PCR conditions
- eg. EGFR+KRAS or EGFR+BRAF+PIK3CA in one plate
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High Sensitivity, Specificity with small amount of DNA
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As this product is Medical Device, please read 'caution' and 'instruction' before use.
Deliberation number : 2014-I10-26-0183 |
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As this product is Medical Device, please read 'caution' and 'instruction' before use.
Deliberation number : 2013-I10-11-2364 |
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| 1 |
Jeong et al., Detection of BRAF(V600E) Mutations in Papillary Thyroid Carcinomas by Peptide Nucleic Acid Clamp Real-Time PCR: A Comparison with Direct Sequencing. Korean J Pathol. 2012;46(1):61-67. |
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| 2 |
Kwon et al., Frequency of KRAS, BRAF, and PIK3CA mutations in advanced colorectal cancers: Comparison of peptide nucleic acid-mediated PCR clamping and direct sequencing in fomalin-flxed, paraffin-embedded tissue. Pathol Res Pract. 2011;207(12):762-768. |
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| 3 |
Shin et al., National Cancer Center, Korea 2002 annual report of the Korea Central Cancer Registry:based on registered data from 139 hospitals. Cancer Res Treat. 2004;36(2):103-114. |
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| 4 |
Kang et al., Prevalence, clinical and ultrasonographic characteristics of thyroid incidentalomas. Thyroid. 2004;14(1):29-33. |
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| 5 |
Meier CA., Thyroid nodules: pathogenesis, diagnosis, and treatment. Baillieres Best Pract Res Clin Endocrinol Metab. 2000;14(4):559-575. |
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As this product is Medical Device, please read 'caution' and 'instruction' before use.
Deliberation number : 2013-I10-11-2364 |
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