PANA qPCR⑩ EML4-ALK |
The Anaplastic Lymphoma Kinase (ALK) gene is frequently involved in rearrangements that lead to gene fusions in lung cancer. Its fusion partner, Echinoderm Microtubule-associated protein-Like 4 (EML4) is often found in Non-Small-Cell Lung Cancer (NSCLC). The recombinant EML4-ALK activates the receptor tyrosine kinase’s downstream signaling pathway, which includes PI3K/AKT, leading to carcinogenesis. It has been reported that the presence of the EML4-ALK fusion gene is correlated with the efficacy of ALK-targeted therapy. PANA qPCR⑩ EML4-ALK kit is the real-time PCR based and provides the simple multiplex test to detect the commonly found EML4-ALK subtypes.
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PANA qPCR⑩ Technology |
PANA qPCR⑩ technology uses a sequence-specific PNA probe with a fluorescent reporter and quencher. The PNA probe is designed to hybridize specifically to the targeted sequence. PANA qPCR⑩ is a powerful tool for multiplexing, allelic discrimination and quantitative analysis with high specificity and sensitivity.
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Products |
Product
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Cat. No.
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Cat. No.
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Size
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Screening |
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PNAQ-3101 |
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25 variants (V1, V6, V3a, V3b, V2,V4, V7, Vnew, V5a, V5b, V8a, V8b, ect.) |
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24 tests |
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Genotyping |
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PNAQ-3201 |
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12 variants (V1, V6, V3a, V3b, V2,V4, V7, Vnew, V5a, V5b, V8a, V8b) |
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10 tests |
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Features |
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PANA qPCR⑩ EML4-ALK Detection kit |
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PANA qPCR⑩ EML4-ALK Genotyping kit
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Components |
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1 tube reaction
( EML4-ALK fusion gene + Internal control ) |
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4 tube
(12 EML4 –ALK fusion gene + Internal control ) |
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Coverage |
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25 EML4-ALK fusion variants detectable |
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EML4-ALK fusion 12 variants detectable |
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Dye |
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ROX, HEX |
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ROX, HEX, FAM, CY5 |
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Probe |
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Common Probe |
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Specific Probe |
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Sensitivity |
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10^2 copy |
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10^2 copy |
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Assessment |
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EML4-ALK positive or negative |
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Assess each variant |
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Size |
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24 tests / kit |
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10 tests/ kit |
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Validated Specimen
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Compatible Instrument |
- Tissue (Fresh, FFPE)
- Biopsy
- Pleural effusions
- Cytology samples
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- Bio-Rad CFX96
- Roche LC480II
- ABI 7500, 7900, StepOnePlus
- Qiagen Rotor-Gene Q
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