PANA qPCR⑩ EML4-ALK
The Anaplastic Lymphoma Kinase (ALK) gene is frequently involved in rearrangements that lead to gene fusions in lung cancer. Its fusion partner, Echinoderm Microtubule-associated protein-Like 4 (EML4) is often found in Non-Small-Cell Lung Cancer (NSCLC). The recombinant EML4-ALK activates the receptor tyrosine kinase’s downstream signaling pathway, which includes PI3K/AKT, leading to carcinogenesis. It has been reported that the presence of the EML4-ALK fusion gene is correlated with the efficacy of ALK-targeted therapy. PANA qPCR⑩ EML4-ALK kit is the real-time PCR based and provides the simple multiplex test to detect the commonly found EML4-ALK subtypes.
PANA qPCR⑩ Technology
PANA qPCR⑩ technology uses a sequence-specific PNA probe with a fluorescent reporter and quencher. The PNA probe is designed to hybridize specifically to the targeted sequence. PANA qPCR⑩ is a powerful tool for multiplexing, allelic discrimination and quantitative analysis with high specificity and sensitivity.
Products
Product Cat. No. Cat. No. Size

Screening

PNAQ-3101

25 variants  (V1, V6, V3a, V3b, V2,V4, V7, Vnew, V5a, V5b, V8a, V8b, ect.)

24 tests

Genotyping

PNAQ-3201

12 variants (V1, V6, V3a, V3b, V2,V4, V7, Vnew, V5a, V5b, V8a, V8b)

10 tests
Features
  PANA qPCR⑩ EML4-ALK Detection kit PANA qPCR⑩ EML4-ALK Genotyping kit

Components

1 tube reaction
( EML4-ALK fusion gene + Internal control )

4 tube
(12 EML4 –ALK fusion gene + Internal control )

Coverage

25 EML4-ALK fusion variants detectable

EML4-ALK fusion 12 variants  detectable

Dye

ROX, HEX

ROX, HEX, FAM, CY5

Probe

Common Probe

Specific Probe

Sensitivity

10^2 copy

10^2 copy

Assessment

EML4-ALK positive or negative

Assess each variant

Size

24 tests / kit

10 tests/ kit

Validated Specimen
 Compatible Instrument
- Tissue (Fresh, FFPE)
- Biopsy
- Pleural effusions
- Cytology samples 		- Bio-Rad CFX96 - Roche LC480II
- ABI 7500, 7900, StepOnePlus
- Qiagen Rotor-Gene Q