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PNAClamp™ Technology is the PNA-based PCR clamping that selectively amplifies only the mutated target DNA sequence as a minor portion in the mixture with the major wild type DNA sequences. PNAClamp™ Technology takes full advantages of PNA probes that have strong binding affinity, specificity to its complementary strands and property of not being recognized at all by DNA polymerase as primer.
PNAClamp™ Technology can be easily applied to detect only the mutated DNA sequences from the mixture with the wild type DNA sequences by PCR amplification. The wild type DNA fragments cannot be amplified by the PNA probes that are tightly bound, whereas only the mutated DNA sequences can be amplified selectively by PCR amplification.
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| PNAClamp™ Technology needs only one additional step of PNA annealing to the wild type DNA sequences compared to the general real-time PCR reactions with three reactions.
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Hyuni Lee et al. 2007. Peptide Nucleic Acid Synthesis by Novel Amide Formation.
Organic Letters 9(17), 3291-3293 |
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