PANA qPCR™ technology uses a sequence-specific PNA probe with a fluorescent reporter and quencher. The PNA probe is designed to hybridize specifically to a nucleotide region of the target sequence.
In the absence of target, no fluorescence is detected from the reporter due to its physical proximity to the quencher. During the annealing step, the PNA probe binds to its specific target sequence, separating the reporter and quencher such that the reporter is no longer quenched. Unlike TaqMan assays, PNA probe is not hydrolyzed during amplification because its resistant to enzyme degradation. PANA qPCR™ is a powerful tool for multiplexing, allelic discrimination experiments and quantitative analysis with high specificity and sensitivity.