PNA°¡ Àü±âÀû Áß¼ºÀ» ³ªÅ¸³»±â ¶§¹®¿¡ in situ hybridization ½Ã ³ôÀº ±âÁú ƯÀ̼ºÀ» ³ªÅ¸³À´Ï´Ù. PNA probe¸¦ ÀÌ¿ëÇÑ FISH (fluorescent in situ hybridization) ¿¬±¸´Â ÀûÀº ³óµµÀÇ PNA probe¸¸À¸·Îµµ ºü¸¥ hybridization ÀÌ °¡´ÉÇÏ¿© background bindingÀ» °¨¼Ò½ÃÅ´À¸·Î½á, ³ôÀº signal-to-noise ratio¸¦ ³ªÅ¸³»°í mildÇÑ Á¶°Ç¿¡¼­µµ washingÀÌ °¡´ÉÇÕ´Ï´Ù.
ÆÄ³ªÁøÀº telomereÀÇ Á¤·®Àû ºÐ¼®, Áï Á¤È®ÇÑ Å©±â¸¦ ºÐ¼®Çϱâ À§ÇÑ ´Ù¾çÇÑ Çü±¤À» labelingÇÑ PNA probe¸¦ ÇÕ¼ºÇÏ¿© Á¦°øÇص帮°í ÀÖ½À´Ï´Ù.
Cat. No. Á¦Ç°¸í

Quantity

F1001-5 TelC-FAM 5nmole
F1002-5 TelC-Cy3
F1003-5 TelC-Cy5
F1009-5 TelC-FITC
F1005-5 TelG-FAM
F1006-5 TelG-Cy3
F1007-5 TelG-Cy5
F1010-5 TelG-FITC
F2001-5 TelC-TMR
F2002-5 TelG-TMR
¿°»öüÀÇ Centromere¸¦ detectionÇÏ¿© Trisomy, polysomy, breakµî°ú °°Àº ¿°»öüÀÇ ¼ö¿Í °ü·ÃµÈ ¿°»öü ÀÌ»óÀ¯¹«¸¦
È®ÀÎÇÒ ¼öÀÖ½À´Ï´Ù.
Cat. No. Á¦Ç°¸í Quantity
F3003-5 Cent-Cy3 5 nmole
F3006-5 Cent-FAM
1 Kentaro Taemura et al., Dynamic rearrangement of telomeres during spermatogenesis in mice. Developmental Biology. 2005;281:196-207.  
2 Won-Woo Lee et al., Age-related telomere length dynamics in peripheral blood mononuclear cells of healthy cynomolgus monkeys measured by Flow FISH. Immunology. 2002;105:458-465.  
3 Heather Perry et al., Identification of indicator microorganisms using a standardized PNA FISH method. Journal of Microbiological Methods. 2001;47:281-292.  
4 Caifu Chen et al., Single base discrimination of CENP-B repeats on mouse and human chromosomes with PNA-FISH. Mammalian Genome. 1999;10:13-18.  
5 M. Hultdin et al., Telomere analysis by flourescence in situ hybridization and flow cytometry. Nucleic Acids Research. 1998;26(16):3651-3656.  
6 Peter M. Landsdorp et al., Heterogeneity in telomere length of human chromosomes. Human Molecular Genetics. 1996;5(5):685-691.