microRNA (miRNA) was discovered from Caenorhbditis elegans by Dr. V. Ambros at Harvard University in 1993. The discovered genes of ‘lin-4 and let-7’ regulate developmental growth at larvae stages. These RNAs were named as stRNA (small temporal RNA) due to its characteristic expression at specific development stages. In 2001 and 2002, more than 150 RNAs were additionally identified from C. elegans, Drosophila melanogaster and Homo sapiens, and these RNAs were renamed as microRNA.
miRNAs usually consist of 19~25 nucleotides and are known to play roles in gene expression of eukaryotes. Recently, miRNAs are reported for their roles not only in cell growth and differentiation but also in cancers, and becoming intensive research area with rapid growth. miRNAs are expected to contribute in therapy of various diseases including cancer and in diagnostics by understanding their functions and regulatory mechanisms.
Series Product Name Cat. No. Quantity
I series  PNAs⑩ miRNA inhibitor PI-XXXX-25 25 nmole
 FAM-labeled PNAs⑩ miRNA inhibitor PI-XXXX-FAM 5 nmole
N series  PNAs⑩ miRNA inhibitor negative control PN-1001 25 nmole
 FAM-labeled negative control PN-1001-FAM 5 nmole
Panagene provides all types of PNAs⑩ miRNA inhibitors to inhibit miRNAs available from miRBase Sequence Database hosted by the Sanger Institute.(http://microrna.sanger.ac.uk)
Please refer to list of human miRNAs as attached
PNAs⑩ miRNA inhibitor
We dispatch PNAs⑩ miRNA inhibitors available in the stock within 2~3 days after confirmation of purchase order.
PNAs miRNA inhibitor
In order to order PNAs⑩ miRNA inhibitors that are not listed or non-human source, please use the order form as attached.
PNAs⑩ miRNA inhibitor order form
  PNAs⑩ miRNA inhibitors are highly sequence-specific
  PNAs⑩ miRNA inhibitors are more effective than other miRNA inhibitors
  PNAs⑩ miRNA inhibitors can be used without using transfection reagent
  PNAs⑩ miRNA inhibitors have long shelf-life
  PNAs⑩ miRNA inhibitors are resistant and very stable cellular nucleases
  PNAs⑩ miRNA inhibitors have no cytotoxicity
Criteria PNAs⑩ miRNA inhibitor LNA 2’-OMe
miRNA inhibitory activity +++++ +++ +
Specificity for target miRNA +++++ +++ ++
Stability to nuclease degradation +++++ ++ +
Toxicity for cell viability - + -
Transfection reagent Not necessary Necessary Necessary
Storage condition RT -20 ∩ -20 ∩
Concentration +++ + ++
 
1 Lu Jiang et al., Identification and experimental validation of G protein alpha inhibiting activity polypeptide 2 (GNAI2) as a microRNA-138 target in tongue squamous cell carcinoma. Hum Genet. 2011;129(2):189-197.  
2 Song J. et al., MicroRNA-488 suppresses cell migration through modulation of the focal adhesion activity during chondrogenic differentiation of chick limb mesenchymal cells. Cell Biol Int. 2011;35(2):179-185.  
3 Lu Jiang et al., Down regulation of the Rho GTPase signaling pathway is involved in the microRNA-138-mediated inhibition of cell migration and invasion in tongue squamous cell carcinoma. Int. J. Cancer. 2010;127:505-512.  
4 Lu Jiang et al., MicroRNA-7 targets IGF1R (insulin-like growth factor 1 receptor) in tongue squamous cell carcinoma cells. Biochem. J. 2010;432:199-205.  
5 Kim D. et al., MicroRNA-221 Regulates Chondrogenic Differentiation through Promoting Proteosomal Degradation of Slug by Targeting Mdm2. J. Biol. Chem. 2010;285(35):26900-26907.  
6 Sarah E. et al., miR-802 Regulates Human Angiotensin II Type 1 Receptor Expression in Intestinal Epithelial C2BBe1 Cells. Am J Physiol Gastrointest Liver Physiol. 2010;299(3):G632-642.  
7 Oh SY et al., PNA-Based Antisense Oligonucleotides for MicroRNAs Inhibition in the Absence of a Transfection Reagent. Oligonucleotides. 2010;20(5):225-230.  
8 Fabani MM et al., Efficient inhibition of miR-155 function in vivo by peptide nucleic acids. Nucleic Acids Res. 2010;38(13):4466-4475.  
9 Oh SY et al., A highly effective and long-lasting inhibition of miRNAs with PNA-based antisense oligonucleotides. Mol Cells. 2009;28:341-345.  
10 Martin M. et al., miR-122 targeting with LNA/2’-O-methyl oligonucleotide mixmers, peptide nucleic acids (PNA), and PNA-peptide conjugates. RNA. 2008;14:336-346.