Welcome > News > °øÁö»çÇ×  
 
 
E
 
±Ûº¸±â
¼º¸í °ü¸®ÀÚ     (Date : 2009-10-19 10:55:43)
Á¦¸ñ [ÆijªÁø ³í¹®¹ßÇ¥] A highly effective and long-lasting inhibition of miRNAs with PNA-based antisense oligonucleotides.
³»¿ë

Mol Cells. 2009 Sep 30. [Epub ahead of print]

A highly effective and long-lasting inhibition of miRNAs with PNA-based antisense oligonucleotides.

Oh SY, Ju Y, Park H.

Panagene Inc., Daejeon, 305-345, Korea.

MiRNAs are non-coding RNAs that play a role in the regulation of major processes. The inhibition of miRNAs using antisense oligonucleotides (ASOs) is a unique and effective technique for the characterization and subsequent therapeutic targeting of miRNA function. Recent advances in ASO chemistry have been used to increase both the resistance to nucleases and the target affinity and specificity of these ASOs.Peptide nucleic acids (PNAs) are artificial oligonucleotides constructed on a peptide-like backbone. PNAs have a stronger affinity and greater specificity to DNA or RNA than natural nucleic acids and are resistant to nucleases, which is an essential characteristic for a miRNA inhibitor that will be exposed to serum and cellular nucleases.For increasing cell penetration, PNAs were conjugated with cell penetrating peptides (CPPs) at N-terminal. Among the tested CPPs, Tat-modified peptide-conjugated PNAs have most effective function for miRNA inhibition. PNA-based ASO was more effective miRNA inhibitor than other DNA-based ASOs and did not show cytotoxicity at concentration up to 1,000 nM. The effects of PNA-based ASOs were shown to persist for 9 days. Also, PNA-based ASOs showed considerable stability at storage temperature. These results suggest that PNA-based ASOs are more effective ASOs of miRNA than DNA-based ASOs and PNA-based ASO technology, compared with other technologies used to inhibit miRNA activity can be an effective tool for investigating miRNA functions.


PMID: 19812898 [PubMed - as supplied by publisher]

Click to paper


 

 ¹øÈ£   Á¦¸ñ ÀÛ¼ºÀÚ ³¯Â¥ ÆÄÀÏ Á¶È¸
   28   ³»ºÎÁ¤º¸°ü¸®±ÔÁ¤(°³Á¤) ¾È³» ÆijªÁø17-09-07 1098
   27   ¿ÜºÎȸ°è°¨»çÀÎ ¼±ÀÓ ÆijªÁø17-05-02 1069
   26   [Áß¿ä°øÁö] Á¦41±â Á¤±âÁÖÁÖÃÑȸ ¼ÒÁý .. ÆijªÁø17-03-16 1075
   25           ³»ºÎÁ¤º¸°ü¸®±ÔÁ¤ ¾È³» ÆijªÁø16-07-18 1101
   24           [Áß¿ä°øÁö] Á¦40±â Á¤±âÁÖÁÖÃÑȸ ¼ÒÁý .. ÆijªÁø16-03-11 3572
   23           [°øÁö] ¾Æ½ºÆ®¶óÁ¦³×Ä« Hands on Work.. ÆijªÁø15-07-22 1094
   22           ½ÅÁ¦Ç°¸í »ç³»°ø¸ð ÆijªÁø14-08-20 1076
   21           PIK3CA µ¹¿¬º¯ÀÌ °ËÃâ Å°Æ® Ãâ½Ã! °ü¸®ÀÚ11-03-08 1072
   20           [GEN ±¤°í]PNAClamp MD Kit °ü¸®ÀÚ10-10-12 1081
   19           [GEN ±¤°í]HPV Genotyping Ch.. °ü¸®ÀÚ10-10-12 1106
   18           ¢ßÆijªÁø, HPV ÆǺ°¿ë PNA À¯ÀüÀÚĨ.. °ü¸®ÀÚ10-04-27 1090
   17           ÆijªÁø, ÀڱðæºÎ¾Ï ¿øÀÎ ¹ÙÀÌ·¯½º À¯Àü.. °ü¸®ÀÚ09-12-29 1092
   16           »õÇØ º¹ ¸¹ÀÌ ¹ÞÀ¸¼¼¿ä.. °ü¸®ÀÚ09-12-29 1098
   15           [ÆÄ°ÝÇÒÀÎ] miRNA ¹ßÇöºÐ¼®¼­ºñ½º °¡.. °ü¸®ÀÚ09-11-05 1086
¢º           [ÆijªÁø ³í¹®¹ßÇ¥] A highly effect.. °ü¸®ÀÚ09-10-19 4512
   13           Çѱ¹¹Ì»ý¹°»ý¸í°øÇÐȸ ¼Ò½ÄÁö¿¡ ÆijªÁø ¼Ò.. °ü¸®ÀÚ09-09-30 1083
   12           PNAs miRNA inhibitorÀÇ 3D .. °ü¸®ÀÚ09-09-08 1065
   11           Korea's Panagene Adds miR.. °ü¸®ÀÚ09-08-12 1084
   10           FAM-labeled PNAs miRNA in.. °ü¸®ÀÚ09-07-30 1081
   9           Fmoc monomer °¡°Ý ÀÎÇÏ! °ü¸®ÀÚ09-07-03 3583
 

< 1 2 >