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파나진은 HPV의 genotyping을 위한 PNA-based microarray (PANArrayTM HPV)를 개발하였고, 최근 식약청 등록을 위한 임상 실험도 완료하였습니다.
또한, 이에 관한 논문을 2009년 4월, Journal of clinical microbiology에 게재하였습니다.
더 자세한 정보는 아래의 논문을 참고하시고, 이와 관련하여 궁금하신 점이 있으시면 info@panagene.com으로 연락주시면 성심성의껏 답변드리겠습니다.
JOURNAL OF CLINICAL MICROBIOLOGY, June 2009, p. 1785–1790
Peptide Nucleic Acid-Based Array for Detecting and Genotyping
Human Papillomaviruses
Jae-jin Choi, Chunhee Kim, and Heekyung Park*
PANAGENE Inc., Daejeon 305-510, Korea
* To whom correspondence should be addressed. Email: hkpark@panagene.com
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Abstract
We describe a novel array for accurate and reliable genotyping of human papillomavirus (HPV) using peptide nucleic acid (PNA) probes. In order to exploit the superior hybridization properties of PNA with target HPV DNAs, we developed a novel PNA array (PANArray HPV). PANArray HPV enables the detection and genotyping of HPVs using 32 type-specific PNA capture probes for medically important HPVs. All tested HPV types showed highly unique hybridization patterns with type-specific PNA probes. PNA array results showed stable specificities and sensitivities after up to 13 months of storage at room temperature. Also, we demonstrated the superior specificity, sensitivity, and stability of PNA arrays for HPV genotyping. We compared the genotyping results of the PNA array to sequencing with MY09/11 PCR products derived from 72 clinical samples. The results showed excellent agreement between the PNA array and sequencing, except for samples reflecting multiple infections. The results from the PNA array were compared with those of type-specific PCR when discrepant results occurred owing to multiple infections. The results for the PNA array matched those of type-specific PCR in all cases. Newly developed PNA arrays show excellent specificity and sensitivity and long shelf life. Our results suggest that the PNA array represents a reliable alternative to conventional DNA arrays for HPV genotyping, as well as for diagnostics.
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