Welcome > Support > Âü°íÀÚ·á  
 
 
E
 
±Ûº¸±â
¼º¸í ÆÄ³ªÁø     (Date : 2010-05-19 15:31:36)
Á¦¸ñ [³í¹®] [ÆÄ³ªÁø ³í¹®¹ßÇ¥]Highly Sensitive PNA Array Platform Technology for Single Nucleotide Mismatch Discrimination
³»¿ë

PNAÀÇ ÀåÁ¡ ÁßÀÇ Çϳª´Â SNP¸¦ detectionÇÒ ¼ö ÀÖ´Ù´Â °ÍÀÔ´Ï´Ù.
(ÁÖ)ÆÄ³ªÁøÀº SNPÀÇ ´ëÇ¥ÀûÀÎ ¿¹·Î¼­ drug-resistant HBV¸¦ °ËÃâÇϱâ À§ÇÑ microarray¸¦ °³¹ßÇÏ¿´½À´Ï´Ù. ÀÌ¿Í °ü·ÃµÈ ÀÚ¼¼ÇÑ ³»¿ëÀº ¾Æ·¡ÀÇ ³í¹®À» Âü°íÇϼ¼¿ä.



J Microbiol Biotechnol.
2010 Feb;20(2):287-93.

Highly sensitive PNA Array Platform Technology for Single Nucleotide Mismatch Discrimination.

Choi JJ, Jang M, Kim J, Park H.
Panagene Inc., Daejeon 305-510, Korea.

Abstract

Reliable discrimination of single nucleotide mismatch was demonstrated using arrays with peptide nucleic acid (PNA) probes. Newly developed PNA probes immobilization method and hybridization conditions for PNA arrays gave excellent specificity and sensitivity. And we compared the specificity, sensitivity, and stability obtained with the PNA and DNA arrays in discriminating single nucleotide mismatches. PNA arrays had superior perfect match-to-mismatch signal ratios and sensitivities. The relative signal intensities of mismatch PNA probes ranged from 1.6% to 12.1% of the perfect match PNA probes. These results demonstrated that the PNA arrays were 2.0 to 37.3 times more specific and about 10 times more sensitive than DNA arrays. A PNA array showed the same specificity and sensitivity after 12-month storage at room temperature.


Link to paper => Click


 

 ¹øÈ£   Á¦¸ñ ÀÛ¼ºÀÚ ÆÄÀÏ Á¶È¸
¢º           [³í¹®] [ÆÄ³ªÁø ³í¹®¹ßÇ¥]Highly Sensitive.. ÆÄ³ªÁø 4619
   28           [³í¹®] [ÆÄ³ªÁø PNA »ç¿ë] Membrane-base.. ÆÄ³ªÁø 6345
   27           [³í¹®] [PNA FISH] Rapid detectio.. ÆÄ³ªÁø 1308
   26           [³í¹®] [PNA FISH]Detection of St.. ÆÄ³ªÁø 62916
   25           [³í¹®] Handy and prompt DNA sepa.. ÆÄ³ªÁø 44521
   24           [³í¹®] Frequency of and variable.. ÆÄ³ªÁø 1250
   23           [³í¹®] Prospects for antisense p.. ÆÄ³ªÁø 39384
   22           [³í¹®] Sequence-specific bacteri.. ÆÄ³ªÁø 36937
   21           [³í¹®] Carrier PNA for shRNA del.. ÆÄ³ªÁø 21732
   20           [³í¹®] [Globin reduction PNA] À¯.. ÆÄ³ªÁø 17362
   19           [³í¹®] [Antiviral research] Inhi.. ÆÄ³ªÁø 3818
   18           [³í¹®] [PNA FISH]Development and.. ÆÄ³ªÁø 4553
   17           [³í¹®] [ÆÄ³ªÁø ³í¹®¹ßÇ¥] A Novel PNA bas.. ÆÄ³ªÁø 8618
   16           [³í¹®] rhinoviruses¿Í enterovirus.. ÆÄ³ªÁø 33503
   15           [³í¹®] PNAÀÇ delivery Çâ»óÀ» À§ÇÑ CPP.. ÆÄ³ªÁø 17005
   14           [³í¹®] ÀûÁ¶ ¿øÀÎ Á¶·ùÀÇ µ¿Á¤: PNA FISH ÀÌ.. ÆÄ³ªÁø 22409
   13           [³í¹®] Èí¿¬ÀÚÀÇ Æó¾Ï ¹ßº´ À§Çè ÆÄ³ªÁø 1222
   12           [³í¹®] PNA °ü·Ã ³í¹® (2001~2007. Nov.. ÆÄ³ªÁø 1241
   11           [³í¹®] PNA °ü·Ã ³í¹® (2008³â) ÆÄ³ªÁø 1166
   10           [³í¹®] PNA ÇÕ¼º ¹æ¹ý ÆÄ³ªÁø 1407
 

< 1 2 3 >