Welcome > Support > Âü°íÀÚ·á  
 
 
E
 
±Ûº¸±â
¼º¸í ÆijªÁø     (Date : 2010-12-09 09:18:42)
Á¦¸ñ [³í¹®] [ÆijªÁø ³í¹®¹ßÇ¥] PNA-mediated Real-Time PCR Clamping for Detection of EGFR Mutations
³»¿ë

Bull. Korean Chem. Soc. 2010, Vol. 31, No. 12 3525
DOI 10.5012/bkcs.2010.31.12.3525


PNA-mediated Real-Time PCR Clamping for Detection of EGFR Mutations

Jae-jin Choi, Minhey Cho, Miae Oh, Hyunsun Kim, Min-seock Kil, and Heekyung Park

Panagene, Inc. , Daejeon, Korea.

Abstract

Tyrosine kinase inhibitors (TKIs) are currently used in the treatment of patients with advanced lung cancer. Recent studies on non-small cell lung cancer have shown that some patients carry somatic mutations in the epidermal growth factor receptor (EGFR) gene. Such mutations correlate with the effectiveness of certain TKIs. To detect a small amount of mutant EGFR among an abundance of wild-type EGFR, we have developed a highly sensitive and simple method using PNAmediated real-time PCR clamping. The PNA-mediated real-time PCR clamping enables detection of EGFR mutants down to approximately 1% mutant -to- wild type. The total assay time was short as it required only 2.0 hr. Thus, PNAmediated real-time PCR clamping can easily be applied to clinical samples for identification of DNA carrying EGFR mutations and also appear to be the best assay to detect somatic mutations.

* ÀÚ¼¼ÇÑ ³»¿ëÀº ÷ºÎÆÄÀÏ ÂüÁ¶ ¹Ù¶ø´Ï´Ù.


 

 ¹øÈ£   Á¦¸ñ ÀÛ¼ºÀÚ ÆÄÀÏ Á¶È¸
   49           [³í¹®] [2016]Comparison of EGFR .. ÆijªÁø 30467
   48           [³í¹®] [2015]IDH Mutation Analys.. ÆijªÁø 65535
   47           [³í¹®] [2015]Low frequency of KR.. ÆijªÁø 29372
   46           [³í¹®] [2015]Simultaneous genoty.. ÆijªÁø 6231
   45           [³í¹®] [2014]Simultaneous diagno.. ÆijªÁø 21137
   44           [³í¹®] [2014]KRAS Mutation Detec.. ÆijªÁø 8577
   43           [³í¹®] [2013]Detection of EGFR m.. ÆijªÁø 1336
   42           [³í¹®] [2013]Detection and compa.. ÆijªÁø 5978
   41           [³í¹®] [2013]Detection of BRAF V.. ÆijªÁø 6261
   40           [³í¹®] [2013]Comparison of Direc.. ÆijªÁø 25447
   39           [³í¹®] [Microarray]Peptide nucle.. ÆijªÁø 14485
   38           [³í¹®] [Clamp]Rapid and Sensitiv.. ÆijªÁø 22404
   37           [³í¹®] [ÆijªÁø Á¦Ç°»ç¿ë ³í¹®] EGFR µ¹¿¬º¯.. ÆijªÁø 3550
   36           [³í¹®] [ÆijªÁø Á¦Ç°»ç¿ë ³í¹®] Development .. ÆijªÁø 14322
   35           [³í¹®] [ÆijªÁø Á¦Ç°»ç¿ë ³í¹®] JHDM3A modul.. ÆijªÁø 1329
   34           [³í¹®] [PNA Chip vs DNA Chip ÀÓ»ó.. ÆijªÁø 4191
¢º           [³í¹®] [ÆijªÁø ³í¹®¹ßÇ¥] PNA-mediated Re.. ÆijªÁø 8988
   32           [³í¹®] [ÆijªÁø Á¦Ç°»ç¿ë ³í¹®] ºñ¼Ò¼¼Æ÷Æó¾Ï¿¡.. ÆijªÁø 14640
   31           [³í¹®] [ÆijªÁø ³í¹® ¹ßÇ¥] PNA-Based Anti.. ÆijªÁø 1333
   30           [³í¹®] [ÆijªÁø ³í¹®¹ßÇ¥]Peptide nucleic .. ÆijªÁø 4055
 

< 1 2 3 >