Welcome > Support > Âü°íÀÚ·á  
 
 
E
 
±Ûº¸±â
¼º¸í ÆijªÁø     (Date : 2010-12-09 09:18:42)
Á¦¸ñ [³í¹®] [ÆijªÁø ³í¹®¹ßÇ¥] PNA-mediated Real-Time PCR Clamping for Detection of EGFR Mutations
³»¿ë

Bull. Korean Chem. Soc. 2010, Vol. 31, No. 12 3525
DOI 10.5012/bkcs.2010.31.12.3525


PNA-mediated Real-Time PCR Clamping for Detection of EGFR Mutations

Jae-jin Choi, Minhey Cho, Miae Oh, Hyunsun Kim, Min-seock Kil, and Heekyung Park

Panagene, Inc. , Daejeon, Korea.

Abstract

Tyrosine kinase inhibitors (TKIs) are currently used in the treatment of patients with advanced lung cancer. Recent studies on non-small cell lung cancer have shown that some patients carry somatic mutations in the epidermal growth factor receptor (EGFR) gene. Such mutations correlate with the effectiveness of certain TKIs. To detect a small amount of mutant EGFR among an abundance of wild-type EGFR, we have developed a highly sensitive and simple method using PNAmediated real-time PCR clamping. The PNA-mediated real-time PCR clamping enables detection of EGFR mutants down to approximately 1% mutant -to- wild type. The total assay time was short as it required only 2.0 hr. Thus, PNAmediated real-time PCR clamping can easily be applied to clinical samples for identification of DNA carrying EGFR mutations and also appear to be the best assay to detect somatic mutations.

* ÀÚ¼¼ÇÑ ³»¿ëÀº ÷ºÎÆÄÀÏ ÂüÁ¶ ¹Ù¶ø´Ï´Ù.


 

 ¹øÈ£   Á¦¸ñ ÀÛ¼ºÀÚ ÆÄÀÏ Á¶È¸
   49           [³í¹®] [2016]Comparison of EGFR .. ÆijªÁø 28911
   48           [³í¹®] [2015]IDH Mutation Analys.. ÆijªÁø 65535
   47           [³í¹®] [2015]Low frequency of KR.. ÆijªÁø 29249
   46           [³í¹®] [2015]Simultaneous genoty.. ÆijªÁø 6107
   45           [³í¹®] [2014]Simultaneous diagno.. ÆijªÁø 21012
   44           [³í¹®] [2014]KRAS Mutation Detec.. ÆijªÁø 8321
   43           [³í¹®] [2013]Detection of EGFR m.. ÆijªÁø 1213
   42           [³í¹®] [2013]Detection and compa.. ÆijªÁø 5854
   41           [³í¹®] [2013]Detection of BRAF V.. ÆijªÁø 6137
   40           [³í¹®] [2013]Comparison of Direc.. ÆijªÁø 25324
   39           [³í¹®] [Microarray]Peptide nucle.. ÆijªÁø 14361
   38           [³í¹®] [Clamp]Rapid and Sensitiv.. ÆijªÁø 22281
   37           [³í¹®] [ÆijªÁø Á¦Ç°»ç¿ë ³í¹®] EGFR µ¹¿¬º¯.. ÆijªÁø 3426
   36           [³í¹®] [ÆijªÁø Á¦Ç°»ç¿ë ³í¹®] Development .. ÆijªÁø 14197
   35           [³í¹®] [ÆijªÁø Á¦Ç°»ç¿ë ³í¹®] JHDM3A modul.. ÆijªÁø 1205
   34           [³í¹®] [PNA Chip vs DNA Chip ÀÓ»ó.. ÆijªÁø 4067
¢º           [³í¹®] [ÆijªÁø ³í¹®¹ßÇ¥] PNA-mediated Re.. ÆijªÁø 8862
   32           [³í¹®] [ÆijªÁø Á¦Ç°»ç¿ë ³í¹®] ºñ¼Ò¼¼Æ÷Æó¾Ï¿¡.. ÆijªÁø 14515
   31           [³í¹®] [ÆijªÁø ³í¹® ¹ßÇ¥] PNA-Based Anti.. ÆijªÁø 1209
   30           [³í¹®] [ÆijªÁø ³í¹®¹ßÇ¥]Peptide nucleic .. ÆijªÁø 3931
 

< 1 2 3 >