Welcome > Support > Âü°íÀÚ·á  
 
 
E
 
±Ûº¸±â
¼º¸í ÆijªÁø     (Date : 2011-01-25 19:08:24)
Á¦¸ñ [³í¹®] [ÆijªÁø Á¦Ç°»ç¿ë ³í¹®] Development of peptide nucleic acid (PNA) microarray for identification of Panax species based on the nuclear ribosomal internal transcribed spacer (ITS) and 5.8S rDNA regions
³»¿ë

* Genes & Genomics¿¡¼­ ÀúÈñ Á¦Ç°ÀÎ PNA microarray¸¦ »ç¿ëÇÏ¿© ¹ßÇ¥ÇÑ ³í¹®ÀÔ´Ï´Ù.


Genes & Genomics
Volume 32, Number 5, 463-468
, DOI: 10.1007/s13258-010-0040-7

Development of peptide nucleic acid (PNA) microarray for identification of Panax species based on the nuclear ribosomal internal transcribed spacer (ITS) and 5.8S rDNA regions

Jei-wan Lee, Kyung-Hwan Bang, Jae-Jin Choi, Jong-Wook Chung, Jeong-Hoon Lee, Ick-Hyun Jo, A-Yeon Seo, Young-Chang Kim, OK-Tae Kim and Seon-Woo Cha

Abstract
This study describes the identification of Panax species using a peptide nucleic acid (PNA) microarray. P. ginseng, P. quienquefolius, and P. japonicus were distinguished from each other using 5 PNA probes designed based on three single nucleotide polymorphisms (SNPs) detected in internal transcribed spacer (ITS) and 5.8S rDNA regions. Signal intensity comparison between PNA and DNA microarrays revealed that the PNA microarray provides a significantly more stable and specific fluorescent signal intensity than the DNA microarray. Three Panax species identified by the PNA microarray were denoted as barcode numbers depending on their fluorescent signal patterns of each species using 5 PNA probes (PG-ITS-116, PG-ITS-414-1, PG-ITS-414-2, PG-ITS-425-1, and PG-ITS-425-2). P. ginseng, P. quinquefolius, and P. japonicus were denoted as ¡®11010¡¯, ¡®00202¡¯ and ¡®00000¡¯, respectively. The PNA microarray developed in this study will be useful for legitimizing the distribution of ginseng in domestic and foreign ginseng markets.
 
Keywords Panax ginseng  - Internal transcribed spacer - 5.8S ribosomal DNA - Peptide nucleotide acid - Microarray - single nucleotide polymorphism

* Link - Click to HERE


 

 ¹øÈ£   Á¦¸ñ ÀÛ¼ºÀÚ ÆÄÀÏ Á¶È¸
   49           [³í¹®] [2016]Comparison of EGFR .. ÆijªÁø 28891
   48           [³í¹®] [2015]IDH Mutation Analys.. ÆijªÁø 65535
   47           [³í¹®] [2015]Low frequency of KR.. ÆijªÁø 29227
   46           [³í¹®] [2015]Simultaneous genoty.. ÆijªÁø 6085
   45           [³í¹®] [2014]Simultaneous diagno.. ÆijªÁø 20992
   44           [³í¹®] [2014]KRAS Mutation Detec.. ÆijªÁø 8141
   43           [³í¹®] [2013]Detection of EGFR m.. ÆijªÁø 1192
   42           [³í¹®] [2013]Detection and compa.. ÆijªÁø 5833
   41           [³í¹®] [2013]Detection of BRAF V.. ÆijªÁø 6113
   40           [³í¹®] [2013]Comparison of Direc.. ÆijªÁø 25304
   39           [³í¹®] [Microarray]Peptide nucle.. ÆijªÁø 14339
   38           [³í¹®] [Clamp]Rapid and Sensitiv.. ÆijªÁø 22261
   37           [³í¹®] [ÆijªÁø Á¦Ç°»ç¿ë ³í¹®] EGFR µ¹¿¬º¯.. ÆijªÁø 3403
¢º           [³í¹®] [ÆijªÁø Á¦Ç°»ç¿ë ³í¹®] Development .. ÆijªÁø 14176
   35           [³í¹®] [ÆijªÁø Á¦Ç°»ç¿ë ³í¹®] JHDM3A modul.. ÆijªÁø 1182
   34           [³í¹®] [PNA Chip vs DNA Chip ÀÓ»ó.. ÆijªÁø 4044
   33           [³í¹®] [ÆijªÁø ³í¹®¹ßÇ¥] PNA-mediated Re.. ÆijªÁø 8840
   32           [³í¹®] [ÆijªÁø Á¦Ç°»ç¿ë ³í¹®] ºñ¼Ò¼¼Æ÷Æó¾Ï¿¡.. ÆijªÁø 14493
   31           [³í¹®] [ÆijªÁø ³í¹® ¹ßÇ¥] PNA-Based Anti.. ÆijªÁø 1190
   30           [³í¹®] [ÆijªÁø ³í¹®¹ßÇ¥]Peptide nucleic .. ÆijªÁø 3912
 

< 1 2 3 >