Welcome > Support > Âü°íÀÚ·á  
 
 
E
 
±Ûº¸±â
¼º¸í ÆÄ³ªÁø     (Date : 2016-07-13 10:15:49)
Á¦¸ñ [³í¹®] [2013]Detection of BRAF V600E mutation with thyroid tissue using pyrosequencing: comparison with PNA-clampingand real-time PCR.
³»¿ë

Am J Clin Pathol. 2013 Jun;139(6):759-64. doi: 10.1309/AJCPN3ULH6YWBHPH.

Detection of BRAF V600E mutation with thyroid tissue using pyrosequencing: comparison with PNA-clampingand real-time PCR.

Kang SH1Pyo JYYang SWHong SW.

Author information

1Department of Laboratory Medicine, Chosun University Medical School, Gwangju, Korea.

Abstract

We used pyrosequencing, peptide nucleic acid (PNA)-clamping polymerase chain reaction (PCR), and real-time PCR to detect the BRAF V600E mutation and to investigate the prognostic effect of the BRAF V600E mutation in paraffin block specimens from 100 patients diagnosed with papillary thyroid carcinoma. Positive rates of PNA-clamping PCR, real-time PCR, and pyrosequencing were 66%, 70%, and 68%, respectively. Pyrosequencing and PNA-clamping PCR detected mutant type in a 99:1 (wild-type: mutant) DNA concentration, and PNA-clamping PCR detected mutant type in a 99.5:0.5 DNA concentration. Clamping PCR showed higher κ value than real-time PCR (0.729 vs 0.626). The BRAF V600E mutation was associated with an advanced stage of cancer (P = .045) and was found to be associated with poor prognostic factors. This study suggests that pyrosequencing can be as sensitive as real-time PCR and that PNA-clamping PCR is a sensitive and reliable method to detect the BRAF V600E mutation.


 

 ¹øÈ£   Á¦¸ñ ÀÛ¼ºÀÚ ÆÄÀÏ Á¶È¸
   49           [³í¹®] [2016]Comparison of EGFR .. ÆÄ³ªÁø 30650
   48           [³í¹®] [2015]IDH Mutation Analys.. ÆÄ³ªÁø 65535
   47           [³í¹®] [2015]Low frequency of KR.. ÆÄ³ªÁø 43991
   46           [³í¹®] [2015]Simultaneous genoty.. ÆÄ³ªÁø 6524
   45           [³í¹®] [2014]Simultaneous diagno.. ÆÄ³ªÁø 25604
   44           [³í¹®] [2014]KRAS Mutation Detec.. ÆÄ³ªÁø 11242
   43           [³í¹®] [2013]Detection of EGFR m.. ÆÄ³ªÁø 1487
   42           [³í¹®] [2013]Detection and compa.. ÆÄ³ªÁø 6135
¢º           [³í¹®] [2013]Detection of BRAF V.. ÆÄ³ªÁø 6474
   40           [³í¹®] [2013]Comparison of Direc.. ÆÄ³ªÁø 32186
   39           [³í¹®] [Microarray]Peptide nucle.. ÆÄ³ªÁø 17428
   38           [³í¹®] [Clamp]Rapid and Sensitiv.. ÆÄ³ªÁø 40117
   37           [³í¹®] [ÆÄ³ªÁø Á¦Ç°»ç¿ë ³í¹®] EGFR µ¹¿¬º¯.. ÆÄ³ªÁø 3745
   36           [³í¹®] [ÆÄ³ªÁø Á¦Ç°»ç¿ë ³í¹®] Development .. ÆÄ³ªÁø 30412
   35           [³í¹®] [ÆÄ³ªÁø Á¦Ç°»ç¿ë ³í¹®] JHDM3A modul.. ÆÄ³ªÁø 1533
   34           [³í¹®] [PNA Chip vs DNA Chip ÀÓ»ó.. ÆÄ³ªÁø 15821
   33           [³í¹®] [ÆÄ³ªÁø ³í¹®¹ßÇ¥] PNA-mediated Re.. ÆÄ³ªÁø 14890
   32           [³í¹®] [ÆÄ³ªÁø Á¦Ç°»ç¿ë ³í¹®] ºñ¼Ò¼¼Æ÷Æó¾Ï¿¡.. ÆÄ³ªÁø 23343
   31           [³í¹®] [ÆÄ³ªÁø ³í¹® ¹ßÇ¥] PNA-Based Anti.. ÆÄ³ªÁø 4052
   30           [³í¹®] [ÆÄ³ªÁø ³í¹®¹ßÇ¥]Peptide nucleic .. ÆÄ³ªÁø 4284
 

< 1 2 3 >