Welcome > Support > Âü°íÀÚ·á  
 
 
E
 
±Ûº¸±â
¼º¸í ÆijªÁø     (Date : 2016-07-13 10:15:49)
Á¦¸ñ [³í¹®] [2013]Detection of BRAF V600E mutation with thyroid tissue using pyrosequencing: comparison with PNA-clampingand real-time PCR.
³»¿ë

Am J Clin Pathol. 2013 Jun;139(6):759-64. doi: 10.1309/AJCPN3ULH6YWBHPH.

Detection of BRAF V600E mutation with thyroid tissue using pyrosequencing: comparison with PNA-clampingand real-time PCR.

Kang SH1Pyo JYYang SWHong SW.

Author information

1Department of Laboratory Medicine, Chosun University Medical School, Gwangju, Korea.

Abstract

We used pyrosequencing, peptide nucleic acid (PNA)-clamping polymerase chain reaction (PCR), and real-time PCR to detect the BRAF V600E mutation and to investigate the prognostic effect of the BRAF V600E mutation in paraffin block specimens from 100 patients diagnosed with papillary thyroid carcinoma. Positive rates of PNA-clamping PCR, real-time PCR, and pyrosequencing were 66%, 70%, and 68%, respectively. Pyrosequencing and PNA-clamping PCR detected mutant type in a 99:1 (wild-type: mutant) DNA concentration, and PNA-clamping PCR detected mutant type in a 99.5:0.5 DNA concentration. Clamping PCR showed higher κ value than real-time PCR (0.729 vs 0.626). The BRAF V600E mutation was associated with an advanced stage of cancer (P = .045) and was found to be associated with poor prognostic factors. This study suggests that pyrosequencing can be as sensitive as real-time PCR and that PNA-clamping PCR is a sensitive and reliable method to detect the BRAF V600E mutation.


 

 ¹øÈ£   Á¦¸ñ ÀÛ¼ºÀÚ ÆÄÀÏ Á¶È¸
   49           [³í¹®] [2016]Comparison of EGFR .. ÆijªÁø 28876
   48           [³í¹®] [2015]IDH Mutation Analys.. ÆijªÁø 65535
   47           [³í¹®] [2015]Low frequency of KR.. ÆijªÁø 29214
   46           [³í¹®] [2015]Simultaneous genoty.. ÆijªÁø 6071
   45           [³í¹®] [2014]Simultaneous diagno.. ÆijªÁø 20977
   44           [³í¹®] [2014]KRAS Mutation Detec.. ÆijªÁø 8127
   43           [³í¹®] [2013]Detection of EGFR m.. ÆijªÁø 1178
   42           [³í¹®] [2013]Detection and compa.. ÆijªÁø 5819
¢º           [³í¹®] [2013]Detection of BRAF V.. ÆijªÁø 6101
   40           [³í¹®] [2013]Comparison of Direc.. ÆijªÁø 25289
   39           [³í¹®] [Microarray]Peptide nucle.. ÆijªÁø 14324
   38           [³í¹®] [Clamp]Rapid and Sensitiv.. ÆijªÁø 22245
   37           [³í¹®] [ÆijªÁø Á¦Ç°»ç¿ë ³í¹®] EGFR µ¹¿¬º¯.. ÆijªÁø 3390
   36           [³í¹®] [ÆijªÁø Á¦Ç°»ç¿ë ³í¹®] Development .. ÆijªÁø 14160
   35           [³í¹®] [ÆijªÁø Á¦Ç°»ç¿ë ³í¹®] JHDM3A modul.. ÆijªÁø 1169
   34           [³í¹®] [PNA Chip vs DNA Chip ÀÓ»ó.. ÆijªÁø 4031
   33           [³í¹®] [ÆijªÁø ³í¹®¹ßÇ¥] PNA-mediated Re.. ÆijªÁø 8825
   32           [³í¹®] [ÆijªÁø Á¦Ç°»ç¿ë ³í¹®] ºñ¼Ò¼¼Æ÷Æó¾Ï¿¡.. ÆijªÁø 14480
   31           [³í¹®] [ÆijªÁø ³í¹® ¹ßÇ¥] PNA-Based Anti.. ÆijªÁø 1175
   30           [³í¹®] [ÆijªÁø ³í¹®¹ßÇ¥]Peptide nucleic .. ÆijªÁø 3896
 

< 1 2 3 >