Welcome > Support > Âü°íÀÚ·á  
 
 
E
 
±Ûº¸±â
¼º¸í ÆijªÁø     (Date : 2016-07-13 10:15:49)
Á¦¸ñ [³í¹®] [2013]Detection of BRAF V600E mutation with thyroid tissue using pyrosequencing: comparison with PNA-clampingand real-time PCR.
³»¿ë

Am J Clin Pathol. 2013 Jun;139(6):759-64. doi: 10.1309/AJCPN3ULH6YWBHPH.

Detection of BRAF V600E mutation with thyroid tissue using pyrosequencing: comparison with PNA-clampingand real-time PCR.

Kang SH1Pyo JYYang SWHong SW.

Author information

1Department of Laboratory Medicine, Chosun University Medical School, Gwangju, Korea.

Abstract

We used pyrosequencing, peptide nucleic acid (PNA)-clamping polymerase chain reaction (PCR), and real-time PCR to detect the BRAF V600E mutation and to investigate the prognostic effect of the BRAF V600E mutation in paraffin block specimens from 100 patients diagnosed with papillary thyroid carcinoma. Positive rates of PNA-clamping PCR, real-time PCR, and pyrosequencing were 66%, 70%, and 68%, respectively. Pyrosequencing and PNA-clamping PCR detected mutant type in a 99:1 (wild-type: mutant) DNA concentration, and PNA-clamping PCR detected mutant type in a 99.5:0.5 DNA concentration. Clamping PCR showed higher κ value than real-time PCR (0.729 vs 0.626). The BRAF V600E mutation was associated with an advanced stage of cancer (P = .045) and was found to be associated with poor prognostic factors. This study suggests that pyrosequencing can be as sensitive as real-time PCR and that PNA-clamping PCR is a sensitive and reliable method to detect the BRAF V600E mutation.


 

 ¹øÈ£   Á¦¸ñ ÀÛ¼ºÀÚ ÆÄÀÏ Á¶È¸
   49           [³í¹®] [2016]Comparison of EGFR .. ÆijªÁø 30464
   48           [³í¹®] [2015]IDH Mutation Analys.. ÆijªÁø 65535
   47           [³í¹®] [2015]Low frequency of KR.. ÆijªÁø 29369
   46           [³í¹®] [2015]Simultaneous genoty.. ÆijªÁø 6228
   45           [³í¹®] [2014]Simultaneous diagno.. ÆijªÁø 21134
   44           [³í¹®] [2014]KRAS Mutation Detec.. ÆijªÁø 8574
   43           [³í¹®] [2013]Detection of EGFR m.. ÆijªÁø 1333
   42           [³í¹®] [2013]Detection and compa.. ÆijªÁø 5975
¢º           [³í¹®] [2013]Detection of BRAF V.. ÆijªÁø 6258
   40           [³í¹®] [2013]Comparison of Direc.. ÆijªÁø 25444
   39           [³í¹®] [Microarray]Peptide nucle.. ÆijªÁø 14482
   38           [³í¹®] [Clamp]Rapid and Sensitiv.. ÆijªÁø 22400
   37           [³í¹®] [ÆijªÁø Á¦Ç°»ç¿ë ³í¹®] EGFR µ¹¿¬º¯.. ÆijªÁø 3547
   36           [³í¹®] [ÆijªÁø Á¦Ç°»ç¿ë ³í¹®] Development .. ÆijªÁø 14317
   35           [³í¹®] [ÆijªÁø Á¦Ç°»ç¿ë ³í¹®] JHDM3A modul.. ÆijªÁø 1326
   34           [³í¹®] [PNA Chip vs DNA Chip ÀÓ»ó.. ÆijªÁø 4188
   33           [³í¹®] [ÆijªÁø ³í¹®¹ßÇ¥] PNA-mediated Re.. ÆijªÁø 8984
   32           [³í¹®] [ÆijªÁø Á¦Ç°»ç¿ë ³í¹®] ºñ¼Ò¼¼Æ÷Æó¾Ï¿¡.. ÆijªÁø 14636
   31           [³í¹®] [ÆijªÁø ³í¹® ¹ßÇ¥] PNA-Based Anti.. ÆijªÁø 1330
   30           [³í¹®] [ÆijªÁø ³í¹®¹ßÇ¥]Peptide nucleic .. ÆijªÁø 4051
 

< 1 2 3 >