Welcome > Support > Âü°íÀÚ·á  
 
 
E
 
±Ûº¸±â
¼º¸í ÆijªÁø     (Date : 2016-07-13 10:21:46)
Á¦¸ñ [³í¹®] [2015]Simultaneous genotyping of multiple somatic mutations by using a clamping PNA and PNA detection probes.
³»¿ë

Chembiochem. 2015 Jan 19;16(2):209-13. doi: 10.1002/cbic.201402640. Epub 2014 Dec 22.

Simultaneous genotyping of multiple somatic mutations by using a clamping PNA and PNA detection probes.

Kim YT1Kim JWKim SKJoe GHHong IS.

Author information

Abstract

It has been very difficult to detect and quantify multiple somatic mutations simultaneously in single-tube qPCR. Here, a novel method for simultaneous detection of multiple mutations and a melting curve analysis was developed by using clamping PNA and detection PNA probes. Each PNA probe was designed to have a specific melting temperature by the introduction of γ-PNA monomer. This technique was successfully applied to the detection of six genotypes in two different mutations of K-RAS at the same time. Such simultaneous analysis of an amplified curve and a melting curve in qPCR can be widely used for the early diagnosis of cancer and determining the prognosis of drug treatments.

© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

KEYWORDS:

cancer; gamma-PNA; multiple genotyping; peptide nucleic acid; quantitative PCR; somatic mutations


 

 ¹øÈ£   Á¦¸ñ ÀÛ¼ºÀÚ ÆÄÀÏ Á¶È¸
   49           [³í¹®] [2016]Comparison of EGFR .. ÆijªÁø 28871
   48           [³í¹®] [2015]IDH Mutation Analys.. ÆijªÁø 65535
   47           [³í¹®] [2015]Low frequency of KR.. ÆijªÁø 29209
¢º           [³í¹®] [2015]Simultaneous genoty.. ÆijªÁø 6066
   45           [³í¹®] [2014]Simultaneous diagno.. ÆijªÁø 20972
   44           [³í¹®] [2014]KRAS Mutation Detec.. ÆijªÁø 8122
   43           [³í¹®] [2013]Detection of EGFR m.. ÆijªÁø 1173
   42           [³í¹®] [2013]Detection and compa.. ÆijªÁø 5814
   41           [³í¹®] [2013]Detection of BRAF V.. ÆijªÁø 6095
   40           [³í¹®] [2013]Comparison of Direc.. ÆijªÁø 25284
   39           [³í¹®] [Microarray]Peptide nucle.. ÆijªÁø 14319
   38           [³í¹®] [Clamp]Rapid and Sensitiv.. ÆijªÁø 22240
   37           [³í¹®] [ÆijªÁø Á¦Ç°»ç¿ë ³í¹®] EGFR µ¹¿¬º¯.. ÆijªÁø 3385
   36           [³í¹®] [ÆijªÁø Á¦Ç°»ç¿ë ³í¹®] Development .. ÆijªÁø 14155
   35           [³í¹®] [ÆijªÁø Á¦Ç°»ç¿ë ³í¹®] JHDM3A modul.. ÆijªÁø 1164
   34           [³í¹®] [PNA Chip vs DNA Chip ÀÓ»ó.. ÆijªÁø 4026
   33           [³í¹®] [ÆijªÁø ³í¹®¹ßÇ¥] PNA-mediated Re.. ÆijªÁø 8820
   32           [³í¹®] [ÆijªÁø Á¦Ç°»ç¿ë ³í¹®] ºñ¼Ò¼¼Æ÷Æó¾Ï¿¡.. ÆijªÁø 14475
   31           [³í¹®] [ÆijªÁø ³í¹® ¹ßÇ¥] PNA-Based Anti.. ÆijªÁø 1169
   30           [³í¹®] [ÆijªÁø ³í¹®¹ßÇ¥]Peptide nucleic .. ÆijªÁø 3891
 

< 1 2 3 >