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Á¦¸ñ [³í¹®] [2015]Simultaneous genotyping of multiple somatic mutations by using a clamping PNA and PNA detection probes.
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Chembiochem. 2015 Jan 19;16(2):209-13. doi: 10.1002/cbic.201402640. Epub 2014 Dec 22.

Simultaneous genotyping of multiple somatic mutations by using a clamping PNA and PNA detection probes.

Kim YT1Kim JWKim SKJoe GHHong IS.

Author information

Abstract

It has been very difficult to detect and quantify multiple somatic mutations simultaneously in single-tube qPCR. Here, a novel method for simultaneous detection of multiple mutations and a melting curve analysis was developed by using clamping PNA and detection PNA probes. Each PNA probe was designed to have a specific melting temperature by the introduction of γ-PNA monomer. This technique was successfully applied to the detection of six genotypes in two different mutations of K-RAS at the same time. Such simultaneous analysis of an amplified curve and a melting curve in qPCR can be widely used for the early diagnosis of cancer and determining the prognosis of drug treatments.

© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

KEYWORDS:

cancer; gamma-PNA; multiple genotyping; peptide nucleic acid; quantitative PCR; somatic mutations


 

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