Welcome > Support > Âü°íÀÚ·á  
 
 
E
 
±Ûº¸±â
¼º¸í ÆijªÁø     (Date : 2016-07-13 10:23:30)
Á¦¸ñ [³í¹®] [2016]Comparison of EGFR mutation detection between the tissue and cytology using direct sequencing, pyrosequencing and peptide nucleic acid clamping in lung adenocarcinoma: Korean multicentre study
³»¿ë


QJM. 2016 Mar;109(3):167-73. doi: 10.1093/qjmed/hcv103. Epub 2015 Jun 1.

Comparison of EGFR mutation detection between the tissue and cytology using direct sequencing, pyrosequencing and peptide nucleic acid clamping in lung adenocarcinoma: Korean multicentre study.

Min KW1Kim WS2Jang SJ3Choi YD4Chang S5Jung SH6Kim L7Roh MS8Lee CS9Shim JW10Kim MJ11Lee GK12Korean Cardiopulmonary Pathology Study Group.

Author information

Abstract

BACKGROUND:

The importance of sensitive methods for the detection of epidermal growth factor receptor (EGFR) mutation is emphasized. The aim of this study is to perform comparative and concordance analyses of direct sequencing, pyrosequencing and peptide nucleic acid (PNA)clamping for detecting EGFR gene mutations using archived tissue and cytology specimens.

METHODS:

Samples from a total of 112 cases, which were diagnosed with adenocarcinoma of the lung at nine hospitals in Korea were collected. Using the above three methods, the concordance rates of EGFR mutations in exons 18, 19, 20 and 21 were analysed and validated in comparative tissue and cytology specimens.

RESULTS:

Comparison of EGFR mutation detection between the tissue and cytology had a high concordance rate. The diagnostic performance of pyrosequencing and PNA clamping in tissue was higher than that of direct sequencing as well as cytology. Additionally, among some of the patients who had EGFR wild type by single method, EGFR mutations were detected by other methods. Cytology specimens had a diagnostic performance for the detection of EGFR mutations.

CONCLUSIONS:

Cytology specimens had a diagnostic performance for the detection of EGFR mutations that was comparable to that of tissues. For detecting EGFR mutations, pyrosequencing or PNA clamping was more sensitive than direct sequencing. In EGFR mutation negative patients who are difficult to obtain tissue, repeating test using pyrosequencing or PNA clamping is recommended to improve the detection rate of EGFR mutation than only one, especially in cytology.

© The Author 2015. Published by Oxford University Press on behalf of the Association of Physicians. All rights reserved. For Permissions, please email: journals.permissions@oup.com.


 

 ¹øÈ£   Á¦¸ñ ÀÛ¼ºÀÚ ÆÄÀÏ Á¶È¸
¢º           [³í¹®] [2016]Comparison of EGFR .. ÆijªÁø 28883
   48           [³í¹®] [2015]IDH Mutation Analys.. ÆijªÁø 65535
   47           [³í¹®] [2015]Low frequency of KR.. ÆijªÁø 29220
   46           [³í¹®] [2015]Simultaneous genoty.. ÆijªÁø 6078
   45           [³í¹®] [2014]Simultaneous diagno.. ÆijªÁø 20983
   44           [³í¹®] [2014]KRAS Mutation Detec.. ÆijªÁø 8133
   43           [³í¹®] [2013]Detection of EGFR m.. ÆijªÁø 1185
   42           [³í¹®] [2013]Detection and compa.. ÆijªÁø 5825
   41           [³í¹®] [2013]Detection of BRAF V.. ÆijªÁø 6107
   40           [³í¹®] [2013]Comparison of Direc.. ÆijªÁø 25295
   39           [³í¹®] [Microarray]Peptide nucle.. ÆijªÁø 14331
   38           [³í¹®] [Clamp]Rapid and Sensitiv.. ÆijªÁø 22252
   37           [³í¹®] [ÆijªÁø Á¦Ç°»ç¿ë ³í¹®] EGFR µ¹¿¬º¯.. ÆijªÁø 3396
   36           [³í¹®] [ÆijªÁø Á¦Ç°»ç¿ë ³í¹®] Development .. ÆijªÁø 14168
   35           [³í¹®] [ÆijªÁø Á¦Ç°»ç¿ë ³í¹®] JHDM3A modul.. ÆijªÁø 1176
   34           [³í¹®] [PNA Chip vs DNA Chip ÀÓ»ó.. ÆijªÁø 4037
   33           [³í¹®] [ÆijªÁø ³í¹®¹ßÇ¥] PNA-mediated Re.. ÆijªÁø 8832
   32           [³í¹®] [ÆijªÁø Á¦Ç°»ç¿ë ³í¹®] ºñ¼Ò¼¼Æ÷Æó¾Ï¿¡.. ÆijªÁø 14487
   31           [³í¹®] [ÆijªÁø ³í¹® ¹ßÇ¥] PNA-Based Anti.. ÆijªÁø 1182
   30           [³í¹®] [ÆijªÁø ³í¹®¹ßÇ¥]Peptide nucleic .. ÆijªÁø 3904
 

< 1 2 3 >